| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068312 |
| Chromosome: | chromosome 7 |
| Location: | 2704961 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g331050 | (1 of 1) IPR000104//IPR001487 - Antifreeze protein, type I // Bromodomain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCGCGAGTGCATCACCCCCACGGCGGTCAATAGCCATACTACCTAGTG |
| Internal bar code: | GTTAAACGGTGCGGGATAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2005 |
| LEAP-Seq percent confirming: | 80.9524 |
| LEAP-Seq n confirming: | 85 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTCATGTTGCTTGT |
| Suggested primer 2: | GAGCTGACGACTACTACCGC |