| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068318 |
| Chromosome: | chromosome 5 |
| Location: | 3491225 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233551 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCACACCTGGCCTGCCGCTATTCTGCACACCTCGTCTCCCCTTCCTTG |
| Internal bar code: | GAATCACGGGCGGCGTGTATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 101 |
| LEAP-Seq percent confirming: | 38.4615 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCAGGCCGAACCATAGC |
| Suggested primer 2: | GTTACAGCGTCGGATCGTCT |