Insertion junction: CLIP2.068322_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.068322
Chromosome:chromosome 10
Location:3346179
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g443500 PCD3 Polyketide cyclase/dehydrase; (1 of 4) PF03364 - Polyketide cyclase / dehydrase and lipid transport (Polyketide_cyc) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGAGGCTTGACGCCGGTGCGTTTGGGCGAAAGTGATGTCTGTCCTTGT
Internal bar code:GTTTGCCCCAAAGGCCACGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2164
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTGTGACACCTAAACGGC
Suggested primer 2:CGGCTACACTCGCTTCATCT