| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.068336 |
| Chromosome: | chromosome 8 |
| Location: | 3449056 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378450 | (1 of 1) IPR000104//IPR000679 - Antifreeze protein, type I // Zinc finger, GATA-type | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATTCCTACCCTGTTCGGCGGGAAAAGAACAGGCGGCCCGCCGACCGTG |
| Internal bar code: | TTTCGTGCTCGAGTTACCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCAGGCATGGCTTGGAA |
| Suggested primer 2: | AGAAGATGTTGTGGGCGAGG |