| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068351 |
| Chromosome: | chromosome 7 |
| Location: | 679934 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317300 | ROC78,MAPKKKK1,SIK1 | (1 of 1) PTHR24361:SF268 - SERINE/THREONINE-PROTEIN KINASE 3; Mitogen Activated Protein Kinase Kinase Kinase Kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGTGGTGACGACGGGCGAATGGCTTTACAAGGTAGCGTGGCGTCTCCG |
| Internal bar code: | TCCCTCTTTCGGTCACATCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 68 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGCCATCAAGGTGATCC |
| Suggested primer 2: | CATCTCAATGGCGCTGATGC |