Insertion junction: CLIP2.068365_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.068365
Chromosome:chromosome 6
Location:6126443
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291450 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATACAAGTGTGCAGACCTCCCACGTCAACTCACCCAATTACAAAAAGCT
Internal bar code:CGCAGTATCTCATAGGTGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:83
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGTCTGCTTCTGGGGAG
Suggested primer 2:CAACTGTCCAGCACAACTGC