Insertion junction: CLIP2.068365_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.068365
Chromosome:chromosome 6
Location:6126445
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291450 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACTTCAGGCTCACTGGCGGGGTGTGCTTGTCTCTCGAGGTTTTGCGC
Internal bar code:TGTTGACTACCCCGTCCTGAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1174
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CAACTGTCCAGCACAACTGC
Suggested primer 2:CTTGGTCTGCTTCTGGGGAG