| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068371 |
| Chromosome: | chromosome 12 |
| Location: | 1583699 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487200 | (1 of 5) IPR001680//IPR011047//IPR017986//IPR020472 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat | 5'UTR | |
| Cre12.g487250 | (1 of 1) PF06454 - Protein of unknown function (DUF1084) (DUF1084) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCGTGCACAGACGAACTGCCAGTGCAGCAACCAATCTTCAAACCCGC |
| Internal bar code: | TGTAATGAGATTCTTTGTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3440 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGAGCAGGAGGAAAGG |
| Suggested primer 2: | TACGCTCGATGCCTCCATTC |