| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068373 |
| Chromosome: | chromosome 11 |
| Location: | 1945131 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467779 | (1 of 1) 3.2.1.52 - Beta-N-acetylhexosaminidase / N-acetyl-beta-glucosaminidase | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGGTACGGGGGCAAACCGGTGCATTTACAGGCCGCAGCACGAGCTA |
| Internal bar code: | GCCACGAGCGAGGCCACAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 95.9459 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCCACCACCACATCATTA |
| Suggested primer 2: | ACCCGTCATATCACCCAGGA |