| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068379 |
| Chromosome: | chromosome 10 |
| Location: | 1212036 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426250 | (1 of 1) K14800 - pre-rRNA-processing protein TSR2 (TSR2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGTGTACCAGCCCCTCTTCGTGTCAGTCAGAAAGCAGCTAGCACGC |
| Internal bar code: | AATATGAAGCAGATATAGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 121 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGGTAACCCCTCCCTT |
| Suggested primer 2: | GTTTGGACACACGTCACAGC |