| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068380 |
| Chromosome: | chromosome 4 |
| Location: | 2520828 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g222350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCAAGGCCGCCATCCGAGTTGCCGACCCACAGCTGGAGGCCGTGGCCA |
| Internal bar code: | ATAGGCTAATGGAAAATGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 23.3333 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCAGCAGCCAATCAATCG |
| Suggested primer 2: | AGGGTCTGACGCTGTTGAAG |