| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068383 |
| Chromosome: | chromosome 1 |
| Location: | 5837098 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041850 | (1 of 10) PTHR12270:SF6 - GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE | intron | |
| Cre01.g800102 | (1 of 1) K01411 - nardilysin [EC:3.4.24.61] (NRD1) | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCGTTTGTGTGCTTGTGTGCGTGAGCCATGAAGCACCAGCAAAGGA |
| Internal bar code: | GATTCCCCCGTTGCCACGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 78 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAATGCGAATGTGAGTCC |
| Suggested primer 2: | CCCTCCTCTGACACACACAC |