| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.068383 |
| Chromosome: | chromosome 13 |
| Location: | 1546033 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572900 | THO3 | (1 of 1) 3.4.24.70 - Oligopeptidase A; Oligoendopeptidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGCGCTTCCGCGGCCGCGAGCCCTCCACCAAGCCCCTGTTGCAGCA |
| Internal bar code: | GAGCTATTAGACCTCGCTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1605 |
| LEAP-Seq percent confirming: | 95.2381 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGATTACATGCGCATGC |
| Suggested primer 2: | TGTGCACCTTCTCCCACATC |