| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068388 |
| Chromosome: | chromosome 6 |
| Location: | 8010450 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304750 | (1 of 2) K12486 - stromal membrane-associated protein (SMAP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGAACTGGGGATGCGGATGCGTGGGATAACTACCCTAGGCAGTTTTA |
| Internal bar code: | CATTGCGGTGTTGATCAATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3215 |
| LEAP-Seq percent confirming: | 67.7419 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTGAGGACGGTGTTGTCG |
| Suggested primer 2: | CGTGCCCAACTTGTTGTGAG |