| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068392 |
| Chromosome: | mitogenome |
| Location: | 8451 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreMt.g802342 | nad6,801500,ChrepMp06 | (1 of 1) K03884 - NADH-ubiquinone oxidoreductase chain 6 (ND6); NADH dehydrogenase subunit 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTCTTTAGAGGTGTAGCTTAGTTGGTTAAAGCGCAAGATTCCAAATC |
| Internal bar code: | AAACCCTGGAGGACTGAATTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4186 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 144 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 144 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTTCACCGAGTCCCCAAT |
| Suggested primer 2: | GCTTTGTGCGCTGTTAGCTT |