Insertion junction: CLIP2.068407_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.068407
Chromosome:chromosome 2
Location:2283241
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGCGTTTGCGCGTGTCGCAGGATTGCATTTGTGTGTTTGTGCCAAACA
Internal bar code:GCTGATATTGTGATTGTCAGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2062
LEAP-Seq percent confirming:87.7193
LEAP-Seq n confirming:50
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:57

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTTGACGGCTTGATCATG
Suggested primer 2:ATGCCAGCGACAAGTCATGA