| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068421 |
| Chromosome: | chromosome 2 |
| Location: | 6446932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388500 | (1 of 13) PF09273 - Rubisco LSMT substrate-binding (Rubis-subs-bind) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGTGGAGATGTTCTGAGCTAGTTGAGAACCGTTAAGGGAGTGAGTC |
| Internal bar code: | GTTGAAGGGAGGGGAATGTCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1823 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCAGGCAATGCAGTCAA |
| Suggested primer 2: | CTGCCCCTTTCGGTCTGAAT |