Insertion junction: CLIP2.068425_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.068425
Chromosome:chromosome 11
Location:170914
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre11.g467544 IDI1 Isopentenyl-diphosphate delta-isomerase; (1 of 1) K01823 - isopentenyl-diphosphate delta-isomerase (idi, IDI) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCCCTCCATACTCTGACGCCACCTGTCCCCTCTCACGCCACCCCCGC
Internal bar code:AAAGTGGACCTGGCTGTTTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:510
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCCGTGTTCCTGTTCAGC
Suggested primer 2:AAACAGCGCCAACACAATCC