| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068440 |
| Chromosome: | chromosome 5 |
| Location: | 2654400 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240200 | (1 of 5) IPR001841//IPR013083 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR | |
| Cre05.g240225 | (1 of 1) IPR008754//IPR024079 - Peptidase M43, pregnancy-associated plasma-A // Metallopeptidase, catalytic domain | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTTTGCGCCACGGTTCGTGCGCTGCTTCTCATTGCGGACAAAGGGTA |
| Internal bar code: | ACTATATTGGACTGACAAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2326 |
| LEAP-Seq percent confirming: | 98.5507 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 69 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTGCAGCCGAACTCGTAA |
| Suggested primer 2: | GAGCTGTGTGTGAGGCAGAT |