Insertion junction: CLIP2.068441_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.068441
Chromosome:chromosome 12
Location:4839953
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g523550 UMM11 UbiE/COQ5 methyltransferase family protein; (1 of 1) PTHR10108//PTHR10108:SF752 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED gene_edge/mRNA_edge/5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCATATAAGTACACCACAACAATGGTGCCATCAACTGCGCGCATGCTT
Internal bar code:TCGTTCATGCTAGCTGTTTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:2022
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGTGTTGTGTAAACCGTT
Suggested primer 2:GTGACGATGAACAGCGATGC