| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.068449 |
| Chromosome: | chromosome 16 |
| Location: | 2829976 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g662750 | THB11 | (1 of 6) PTHR10681//PTHR10681:SF118 - THIOREDOXIN PEROXIDASE // SUBFAMILY NOT NAMED; Truncated hemoglobin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTATTGACACGGGGCTAGCGGAGCTGGCTGCCCACGCCCTGCCCGT |
| Internal bar code: | AAAGTTATATACCACACTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1789 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGATGCATGTGTCGCAAG |
| Suggested primer 2: | GCAGACAGATAAAGCAGCGC |