| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068454 |
| Chromosome: | chromosome 1 |
| Location: | 3439580 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022250 | uL3m,MRPL3 | Mitochondrial ribosomal protein L3; (1 of 2) K02906 - large subunit ribosomal protein L3 (RP-L3, MRPL3, rplC) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTACATTCCATAGCAGTCATATATGTGCCGCACAACCATGTACTCGG |
| Internal bar code: | TATATTATAGTCGCTGCGGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 72.7273 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGCTGTGGTTTTCGCGT |
| Suggested primer 2: | TCTTCACGCCCTTCTGAACC |