Insertion junction: CLIP2.068456_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.068456
Chromosome:chromosome 6
Location:3734077
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278162 PHC3 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 3 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCCTACCTAGTGTCAGGATTAGCGTACTGGAGTTTCTGCCCAACCGC
Internal bar code:TGTGGGCGAAACAGGGAACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3594
LEAP-Seq percent confirming:29.0323
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAACAACGCAGGGTCTTG
Suggested primer 2:TCCTTCTACCTGGACGCGTA