Insertion junction: CLIP2.068485_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.068485
Chromosome:chromosome 9
Location:4876390
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g403108 (1 of 1) K16601 - tubulin polyglutamylase TTLL4 [EC:6.-.-.-] (TTLL4) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTTATAAACAATACAGATATTATGGTTTGCTTATGAGAACTACTGTCT
Internal bar code:GCACCGAAAAGTACGGCACTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:158
LEAP-Seq percent confirming:7.14286
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTGACTCTGAACCCGAC
Suggested primer 2:ACCTAGGGGATTGGGGGTAC