| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068487 |
| Chromosome: | chromosome 12 |
| Location: | 4549833 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g521350 | SLY1 | (1 of 1) PTHR11679:SF2 - SEC1 FAMILY DOMAIN-CONTAINING PROTEIN 1; SM/Sec1-family protein, SLY1 homolog | 5'UTR |
| Cre12.g521400 | (1 of 1) 6.3.5.11 - Cobyrinate a,c-diamide synthase (glutamine-hydrolyzing) / Cobyrinic acid a,c-diamide synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCAACGTTGGAAAATAAAGCAAGTAGATTGCGCAATATCCAGACTTAT |
| Internal bar code: | GACCGAACGCTCTTTGTGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1069 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCGCAACCTACACCTAC |
| Suggested primer 2: | ACCTCTCCACCTGATACCCC |