Insertion junction: CLIP2.068501_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.068501
Chromosome:chromosome 6
Location:2362228
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g268100 HTB10 (1 of 27) K11252 - histone H2B (H2B); Histone H2B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACAACCCGCCAACCCACCCCTGCTCCGTGTCAAGGTGGTGTTGAGAAA
Internal bar code:TACTACTCGGTCAGCGTCCGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1758
LEAP-Seq percent confirming:22.6415
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTTTGCCGCATCAACAAC
Suggested primer 2:TGCATGCACCATACACCCAT