| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068505 |
| Chromosome: | chromosome 8 |
| Location: | 1557103 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g365103 | FAP319 | Coiled-Coil Flagellar Associated Protein 319 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGACCCTGCTGGCTGGGAAACGGTGACAACGGTGGTGTTATTGATTGAT |
| Internal bar code: | CCCGTCGTCTTCTTGCTGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1824 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCCAACATCCCAGGATTGC |
| Suggested primer 2: | CACCTTCATTTCGTCGGTGC |