Insertion junction: CLIP2.068533_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.068533
Chromosome:chromosome 1
Location:6809357
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g048800 NAT8 (1 of 36) PF00583 - Acetyltransferase (GNAT) family (Acetyltransf_1); N-acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGGAGGGGCAACCTTTTGGCCTGACTAGAAGGCCCATCAGATGAAAG
Internal bar code:CGCTTTCGCTGTCTCCCATAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1111
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATGCTCCAATTCCGAAG
Suggested primer 2:GAAACGAAGCTTGCCTTGCA