| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068563 |
| Chromosome: | chromosome 7 |
| Location: | 5841780 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353650 | (1 of 29) PF00612 - IQ calmodulin-binding motif (IQ) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGACAGTTAAGCAAACGGAGGTCGATTTTTACAGTTCTTGCTAGTAA |
| Internal bar code: | TGCCAGGTAAGATGAACGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4257 |
| LEAP-Seq percent confirming: | 99.2424 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 132 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCACCTTTCTTGTCCGA |
| Suggested primer 2: | GTAATCTGACGGCGCGTTTC |