| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068566 |
| Chromosome: | chromosome 6 |
| Location: | 5291192 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCGCACACCAGCAGGAGAGGGAGCGGGCGCACAGCGCAGCCTGGCT |
| Internal bar code: | TCCTCGTGCTGAGCTAATGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 874 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCTCACAAACCTGCACA |
| Suggested primer 2: | GCTGTCGAAATCCGGATCCA |