| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068576 |
| Chromosome: | chromosome 10 |
| Location: | 3413172 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444044 | (1 of 39) PTHR13078:SF53 - PROTEIN MAOC-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGAGGGGTTATCTTGGTAGGGCCAACAGCGGACTCGCCACCGGCCT |
| Internal bar code: | CGACGAATTCTGGATCCGGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1402 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTAGGAATGGGTGCTGGTT |
| Suggested primer 2: | GGCAACCCAGCTTCAACTTG |