| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068578 |
| Chromosome: | chromosome 2 |
| Location: | 1288688 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082350 | CUTA1,CUT1 | (1 of 1) K03926 - periplasmic divalent cation tolerance protein (cutA); Copper-binding protein CutA | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCGTTGTTCACCTGCGGCAGCAGGCGCGGCGCCCGCGGGCACAGGGC |
| Internal bar code: | AGGAGCGGCTTTAGCTTGATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3006 |
| LEAP-Seq percent confirming: | 92.7273 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCAGAACTATGTGGGGT |
| Suggested primer 2: | GCTCGTAACTCAGCCCAACT |