| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068584 |
| Chromosome: | chromosome 16 |
| Location: | 4484248 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g667729 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGTCGGTACAGTAACTCCGGCGTTCGACCAGCCATGCACTTCTGCTC |
| Internal bar code: | CTAAAGAGGGGTAAGACGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 500 |
| LEAP-Seq percent confirming: | 38.4615 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCCAGTTGAACAAGCTG |
| Suggested primer 2: | CATACACCTCGGGCTCATCC |