| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068587 |
| Chromosome: | chromosome 6 |
| Location: | 952804 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256400 | 5'UTR | ||
| Cre06.g256420 | (1 of 1) K15196 - transcription factor IIIB 90 kDa subunit (BRF1, GTF3B) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAATTACAGCCGACTTATGCAAACCGGTACAGTTGTACAGCCACATC |
| Internal bar code: | CTACGTAAGCGCGGAGGTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3324 |
| LEAP-Seq percent confirming: | 97.7273 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGCGGATGATTTGGAGC |
| Suggested primer 2: | CCAGGGAGGGCAAGTTAGTG |