| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068590 |
| Chromosome: | chromosome 2 |
| Location: | 4179257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g104050 | 5'UTR | ||
| Cre02.g104100 | (1 of 1) PTHR10209//PTHR10209:SF216 - OXIDOREDUCTASE, 2OG-FE II OXYGENASE FAMILY PROTEIN // 2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGGGGAGCCGTGTATGCGTCTCGCGGCATTACATTGATATGGCCCCA |
| Internal bar code: | TCGATGATTAAAGAGGAAACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3578 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACGTTTGCGGTGTTCATG |
| Suggested primer 2: | AGCAATCGAAATCCAGGCCA |