Insertion junction: CLIP2.068591_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.068591
Chromosome:chromosome 3
Location:6043430
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g190000 PPP14 Phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTTACTTAACCGCATAATGAGCCTGAGAATCCTTTTACGGGCGTCGTG
Internal bar code:AGCGGCGGCCATTGATTAAGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:991
LEAP-Seq percent confirming:4.34783
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:69

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCGCTACAGGATCTGAG
Suggested primer 2:AGGTCCTCGCCTACATCCTT