Insertion junction: CLIP2.068601_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.068601
Chromosome:chromosome 12
Location:7219501
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g559053 CSB50 {"Probable transposon-derived protein of Chlamydomonas-Specific family B; (1 of 20) IPR010095//IPR013083 - Transposase IS605, OrfB, C-terminal // Zinc finger, RING/FYVE/PHD-type 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCAACCGGCCACGTCCGGATTTGCGGGGATGCCAAAGGCCCCCAA
Internal bar code:ACTGCCACATAACGATACCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1929
LEAP-Seq percent confirming:95.8333
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCTGCAAGACAAAGACG
Suggested primer 2:GATCCTCCTCATGGCTGCTC