| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068622 |
| Chromosome: | chromosome 17 |
| Location: | 4706618 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g732802 | (1 of 1) 3.7.1.2 - Fumarylacetoacetase / Fumarylacetoacetate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCACAGCGCCACCGGCACTGGCGCATGCACGGCACGGGCACAGGTAT |
| Internal bar code: | CAACTTCGTTGAGTATTGGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAATCACAGAAGGCGCGAAG |
| Suggested primer 2: | CCTGACTGCCTCCCTCCTAT |