| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068625 |
| Chromosome: | chromosome 13 |
| Location: | 4641502 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603800 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCAATGAGCGGGGAAACAGGCAGTGACGGAGTCTGGTGTGGACGGGCG |
| Internal bar code: | AATCTTTTCTAATTGGAATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3605 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAATGACGCATTCGCCAG |
| Suggested primer 2: | GGACGCCGAAGTACTAGAGC |