| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068631 |
| Chromosome: | chromosome 2 |
| Location: | 5650983 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115700 | intron | ||
| lncRNA_TCONS_00042975 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGGTTAGACAGTGACGGCCAGAATCGGGTTTCAGAACCTTCCGCAAG |
| Internal bar code: | GCGCCCTCAAATTCACTGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1614 |
| LEAP-Seq percent confirming: | 41.1765 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCCGGGAAGAGGTAATCG |
| Suggested primer 2: | TATACTACCCACACCGCCCA |