Insertion junction: CLIP2.068638_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.068638
Chromosome:chromosome 16
Location:5376939
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683250 (1 of 1) PF05832 - Eukaryotic protein of unknown function (DUF846) (DUF846) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACAAGCCACAAAAACGACGGCGTCTTCGCATGCGTGTGCCGTTGGCAG
Internal bar code:CATCCCTCGAGTTGAGTAAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3062
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:45
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTTCTTTGCATGCGATCG
Suggested primer 2:GCACGGTGACCTTACTGACA