| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068661 |
| Chromosome: | chromosome 13 |
| Location: | 1774795 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574800 | CYB5-3,CYB3 | (1 of 3) K00326 - cytochrome-b5 reductase (E1.6.2.2); Cytochrome b5 flavohemoprotein | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTTCAGGCAGCCGTTACTATACTGCAATTCTTGGGGCGCAGACTGCA |
| Internal bar code: | ACACGTCTGCCTTATTTGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4138 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 82 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGCACACACGCTAAGCT |
| Suggested primer 2: | GTGCAGTTCACACAAGCTGG |