| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068670 |
| Chromosome: | chromosome 9 |
| Location: | 5248654 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407100 | MRPS5,uS5m | Mitochondrial ribosomal protein S5; (1 of 1) PTHR13718:SF61 - 28S RIBOSOMAL PROTEIN S5, MITOCHONDRIAL | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGGGTGGCACGGCAGAGAGGCAGTGACACAGAGCTTCTTGGGTGAGC |
| Internal bar code: | GAGCTCTGCAAGAATTTCTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 663 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGACAGGTCCAGCTCTTGC |
| Suggested primer 2: | AATGCCAACGAAGGGGTAGG |