| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068671 |
| Chromosome: | chromosome 10 |
| Location: | 2559117 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g436450 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACCTATACAATGCATGGGACCCATGGGGCGAGCACACGTTCATTGACG |
| Internal bar code: | ATAAGGATCTGGTCCATAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4763 |
| LEAP-Seq percent confirming: | 99.4012 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 167 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGAACGCGGTGATGAAGT |
| Suggested primer 2: | GGGAGTTGGCAGTTGTACGA |