| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068684 |
| Chromosome: | chromosome 17 |
| Location: | 2107743 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g802047 | (1 of 14) IPR002156//IPR012337 - Ribonuclease H domain // Ribonuclease H-like domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGGACTTTAATTGACTGGCGACTGTGGCGACTCGTTGCGAGGTAAAGG |
| Internal bar code: | GAGCAGCTTCTCGTTGTTTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1332 |
| LEAP-Seq percent confirming: | 30.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCGTCCACGTACACCAC |
| Suggested primer 2: | AAGATCTCCACCCCCTCCTC |