Insertion junction: CLIP2.068684_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.068684
Chromosome:chromosome 17
Location:2107745
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g802047 (1 of 14) IPR002156//IPR012337 - Ribonuclease H domain // Ribonuclease H-like domain 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACGCGCGAGGTCGCTTCTCACTCCGGCCGAGTTTTACCTGCTGCCTA
Internal bar code:CGCGCTACCACCCCATTGCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3150
LEAP-Seq percent confirming:92.5926
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:AAGATCTCCACCCCCTCCTC
Suggested primer 2:AACTCGTCCACGTACACCAC