| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068701 |
| Chromosome: | chromosome 3 |
| Location: | 6124202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190550 | (1 of 1) PF08638 - Mediator complex subunit MED14 (Med14) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACCCGAATTGTTCTCGCCTCAGTTGAGCACGCTATACACCAGCTCCA |
| Internal bar code: | GCTGAGAAGTTAGCCGTAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 973 |
| LEAP-Seq percent confirming: | 57.732 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTGTGGAGTGGTGGGAG |
| Suggested primer 2: | TCCCATTACGGACGAGGTCT |