| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.068703 |
| Chromosome: | chromosome 3 |
| Location: | 970244 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146807 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACCATCCCCTCTGCACCACCATACATCAAGCCTACGAATCCTCCAC |
| Internal bar code: | TCGTTGTCGTGGAATAGGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 94 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCCTCCATTCCCTACTCA |
| Suggested primer 2: | TGTTACAGGACACGCACCTC |