| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.068707 |
| Chromosome: | chromosome 6 |
| Location: | 4654189 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278350 | AGD1 | (1 of 1) 1.3.1.78 - Arogenate dehydrogenase (NADP(+)) / TyrAAT2; Arogenate/prephenate dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGCCTGTTAATAGCCCCACTCGCACGCTCACATCCACACACTGCGGC |
| Internal bar code: | CCCTCGGTGTTATCAGGTTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1400 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGAAGGTCCGCATTGGG |
| Suggested primer 2: | GGGACCGTACGATGCAGTAG |