| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068721 |
| Chromosome: | chromosome 5 |
| Location: | 3116964 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre24.g755847 | (1 of 1) PTHR11935//PTHR11935:SF104 - BETA LACTAMASE DOMAIN // METALLO-BETA-LACTAMASE FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCCAGCATGTTCAATCCACAGTCCGGTTGTAGGTCTCTGCCGTGCGG |
| Internal bar code: | AAAGGTAGTTTAGAAATCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1146 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTCGCATAAGACGCAAG |
| Suggested primer 2: | CCAGATGATGCCCTCGTTGT |